Antifungal activity of Cenostigma pluviosum var. peltophoroides extract against Colletotrichum isolates associated with soybean anthracnose: effects on sporulation, conidial germination, and appressorium formation
DOI:
https://doi.org/10.36560/19320262198Palavras-chave:
Infection structures,botanical extracts,antifungal compounds, plant secondary metabolites, sustainable disease managementResumo
Plant-derived compounds have been widely investigated as potential alternatives to synthetic fungicides in plant disease management. This study evaluated the antifungal activity of an ethanolic extract obtained from leaves of Cenostigma pluviosum var. peltophoroides against Colletotrichum spp. isolated from soybean plants affected by anthracnose. The isolates were obtained from soybean fields located in three municipalities in the state of Mato Grosso, Brazil. Antifungal activity was evaluated using in vitro assays, including disk-diffusion and spreading methods, to assess mycelial growth inhibition, mycelial growth rate index, sporulation inhibition, conidial germination, and appressorium formation. The extract showed limited inhibition of mycelial growth but significantly reduced sporulation and strongly inhibited appressorium formation, reaching up to 92.30% inhibition. The spreading method showed greater sensitivity for detecting sporulation inhibition compared with the disk-diffusion method. These results indicate that the extract interferes with important stages of the fungal infection process and may represent a potential source of bioactive compounds for alternative management strategies for soybean anthracnose. Further studies are required to identify the active compounds and evaluate their effectiveness under greenhouse and field conditions.
Referências
BAHIA, M. V.; DAVID, J. C.; DAVID, J. M. Occurrence of biflavones in leaves of Caesalpinia pyramidalis. Química Nova, v. 33, p. 1297–1300, 2010. DOI: https://doi.org/10.1590/S0100-40422010000600015
BALBI-PEÑA, M. I. et al. Controle de Alternaria solani em tomateiro por extratos de Curcuma longa e curcumina – II. Avaliação in vivo. Fitopatologia Brasileira, v. 31, p. 401–404, 2006. DOI: https://doi.org/10.1590/S0100-41582006000400012
BARBIERI, M. C. G. et al. First report of Colletotrichum cliviae causing soybean anthracnose in Brazil. Plant Disease, v. 101, p. 1677, 2017. DOI: https://doi.org/10.1094/PDIS-07-16-0963-PDN
BONALDO, S. M. et al. Fungitoxicidade, atividade elicitora de fitoalexinas e proteção de pepino contra Colletotrichum lagenarium pelo extrato aquoso de Eucalyptus citriodora. Fitopatologia Brasileira, v. 29, p. 128–134, 2004. DOI: https://doi.org/10.1590/S0100-41582004000200002
BONALDO, S. M. et al. Atividades antifúngica e elicitora de fitoalexinas em sorgo e soja por Eucalyptus citriodora. Summa Phytopathologica, v. 33, p. 383–387, 2007. DOI: https://doi.org/10.1590/S0100-54052007000400011
BOUFLEUR, T. R. et al. Soybean anthracnose caused by Colletotrichum species: current status and future prospects. Molecular Plant Pathology, v. 22, p. 393–409, 2021. DOI: 10.1111/mpp.13036. DOI: https://doi.org/10.1111/mpp.13036
CASTRO, R. R. L. Characterization, transmission, cultivar reaction and fungicide sensitivity of Colletotrichum plurivorum associated with soybean anthracnose. 2018. Tese (Doutorado) – Universidade de São Paulo, Piracicaba, 2018.
CAVALHEIRO, M. G. et al. Biological and enzymatic activities of aqueous extract of Caesalpinia ferrea seeds. Revista Brasileira de Farmacognosia, v. 19, p. 586–591, 2009. DOI: https://doi.org/10.1590/S0102-695X2009000400014
CHRISTOPHER, F. et al. Antifungal activity of Leonotis nepetifolia extracts against Colletotrichum species causing bean anthracnose and their phytochemical analysis using LC-MS. Journal of Natural Pesticide Research, v. 6, p. 100057, 2023. DOI: 10.1016/j.napere.2023.100057. DOI: https://doi.org/10.1016/j.napere.2023.100057
COWAN, M. M. Plant products as antimicrobial agents. Clinical Microbiology Reviews, v. 12, p. 564–582, 1999. DOI: https://doi.org/10.1128/CMR.12.4.564
CUNHA, A. C. L. et al. Phytochemical analysis, antioxidant activity and toxicological assay of Caesalpinia peltophoroides. In: CONGRESSO BRASILEIRO DE QUÍMICA, 54., 2014. Anais [...]. 2014.
DAMM, U. et al. The Colletotrichum dracaenophilum, C. magnum and C. orchidearum species complexes. Studies in Mycology, v. 92, p. 1–46, 2019. DOI: https://doi.org/10.1016/j.simyco.2018.04.001
DAYAN, F. E.; CANTRELL, C. L.; DUKE, S. O. Natural products in crop protection. Bioorganic & Medicinal Chemistry, v. 17, p. 4022–4034, 2009. DOI: https://doi.org/10.1016/j.bmc.2009.01.046
DIAS, M. D.; PINHEIRO, V. F.; CAFÉ-FILHO, A. C. Impact of anthracnose on soybean yield under chemical control in northern Brazil. Summa Phytopathologica, v. 42, p. 18–23, 2016. DOI: https://doi.org/10.1590/0100-5405/2114
DOMINGOS, O. S. et al. Anti-inflammatory derivatives from metabolomic screening of Poincianella pluviosa. Molecules, v. 24, p. 4375, 2019. DOI: https://doi.org/10.3390/molecules24234375
FERREIRA, D. F. SISVAR: a computer statistical analysis system. Ciência e Agrotecnologia, v. 35, p. 1039–1042, 2011. DOI: https://doi.org/10.1590/S1413-70542011000600001
HARTMAN, G. L.; MANANDHAR, J. B.; SINCLAIR, J. B. Incidence of Colletotrichum spp. on soybean and weeds in Illinois and pathogenicity of Colletotrichum truncatum. Phytopathology, v. 70, p. 780–782, 1986. DOI: https://doi.org/10.1094/PD-70-780
KHAN, R. A.; SINCLAIR, J. B. Colonization of soybean seed tissues by Colletotrichum truncatum. Plant Disease, v. 75, p. 115–117, 1991. DOI: https://doi.org/10.1094/PD-75-1282
KUSHAHA, T. M. et al. Botanical extracts control the fungal pathogen Colletotrichum boninense in smallholder production of common bean. Phytopathology Research, v. 6, p. 19, 2024. DOI: 10.1186/s42483-024-00235-y. DOI: https://doi.org/10.1186/s42483-024-00235-y
LORENZI, H. Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas do Brasil. Nova Odessa: Instituto Plantarum, 2008.
MELLO, F. E. et al. Sensitivity of Colletotrichum plurivorum and C. truncatum from soybean to SDHI and DMI fungicides. Tropical Plant Pathology, v. 49, p. 83–92, 2024. DOI: https://doi.org/10.1007/s40858-023-00617-1
MORAES, S. R. G.; BONALDO, S. M.; GASPARETO, A. R. R. Geographical distribution, diversity and pathogenicity of Colletotrichum associated with soybean anthracnose in Brazil. Scientific Electronic Archives, v. 13, n. 7, p. 77, 2020. DOI: 10.36560/13720201131. DOI: https://doi.org/10.36560/13720201131
OLIVEIRA, J. A. Effect of fungicide seed treatment on damping-off of cucumber and pepper seedlings. 1991. Dissertação (Mestrado) – Universidade Federal de Lavras, Lavras, 1991.
PEREIRA, M. S. V. et al. Antimicrobial activity of plant extracts from the semi-arid region of Paraíba. Agropecuária Científica no Semiárido, v. 2, p. 37–43, 2006.
PUSZTAHELYI, T.; HOLB, I. J.; PÓCSI, I. Secondary metabolites in fungus–plant interactions. Frontiers in Plant Science, v. 6, p. 573, 2015. DOI: https://doi.org/10.3389/fpls.2015.00573
ROGÉRIO, F. et al. Phylogeny and variability of Colletotrichum truncatum associated with soybean anthracnose in Brazil. Journal of Applied Microbiology, v. 122, p. 402–415, 2017. DOI: https://doi.org/10.1111/jam.13346
ROGÉRIO, F. et al. Multiple resistance of Colletotrichum truncatum from soybean to QoI and MBC fungicides in Brazil. Journal of Phytopathology, v. 172, e13341, 2024. DOI: https://doi.org/10.1111/jph.13341
SANTOS, C. G. T.; ARAÚJO, D. S.; BONALDO, S. M. Sensibilidade de Colletotrichum plurivorum e Colletotrichum truncatum a fungicidas comerciais. Scientific Electronic Archives, v. 16, n. 12, 2023. DOI: 10.36560/161220231824. DOI: https://doi.org/10.36560/161220231824
SILVA, B S. Sensibilidade de isolados de Colletotrichum truncatum da soja a fungicidas. 2018. 109 f. Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo. Passo Fundo, RS, 2018.
SUDIRGA, S. K. et al. Assessment of plants’ leaf extracts for antifungal activity against the fungus Colletotrichum acutatum in chili (Capsicum annuum L.). SABRAO Journal of Breeding and Genetics, v. 55, n. 6, p. 2239–2249, 2023. DOI: 10.54910/sabrao2023.55.6.34. DOI: https://doi.org/10.54910/sabrao2023.55.6.34
SOUZA, J. E. et al. Leaves of Poincianella pluviosa as a renewable source of ellagic acid and phenolic compounds. Journal of the Brazilian Chemical Society, v. 29, 2018.
TAKAKI INO, P. Y.; VALERIANO, I.; GONÇALVES, M. P.; BONALDO, S. M.; MORAES, S. R. G.; MASSOLA JÚNIOR, N. S.; BOUFLEUR, T. R. First report of Colletotrichum chlorophyti causing soybean anthracnose in Brazil. Plant Disease, v. 108, n. 9, p. 2926, 2024. DOI: 10.1094/PDIS-01-24-0242-PDN. DOI: https://doi.org/10.1094/PDIS-01-24-0242-PDN
TIKAMI, Í. et al. Survival of Colletotrichum truncatum as microsclerotia in soil. Plant Disease, v. 107, p. 2460–2466, 2023. DOI: https://doi.org/10.1094/PDIS-08-22-1891-RE
TRIPATHI, P.; DUBEY, N. K. Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruits and vegetables. Postharvest Biology and Technology, v. 32, p. 235–245, 2004. DOI: https://doi.org/10.1016/j.postharvbio.2003.11.005
YANG, H. C.; HAUDENSHIELD, J. S.; HARTMAN, G. L. Colletotrichum incanum sp. nov., a curved-conidial species causing soybean anthracnose in the United States. Mycologia, v. 106, n. 1, p. 32–42, 2014. DOI: https://doi.org/10.3852/13-174 DOI: https://doi.org/10.3852/13-013
YANG, H. C.; HARTMAN, G. L. Anthracnose. In: HARTMAN, G. L.; RUPE, J.; SIKORA, E. J. (ed.). Compendium of Soybean Diseases and Pests. St. Paul: APS Press, 2016. p. 31–34. DOI: https://doi.org/10.1094/9780890544754
ZANIN, J. L. B. et al. The genus Caesalpinia L. (Caesalpiniaceae): phytochemical and pharmacological characteristics. Molecules, v. 17, p. 7887–7902, 2012. DOI: https://doi.org/10.3390/molecules17077887
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