Selecting phosphate solubilizing bacteria from suine biofertilizer

Authors

  • Humberto Franco Shiomi Universidade Federal do Mato Grosso
  • Daniele Cristina Costa Sabino Universidade Federal do Mato Grosso
  • Débora Regina Serbai Universidade Federal do Mato Grosso
  • Júlia Dal Pai Busanelo Universidade Federal do Mato Grosso
  • Carlos Vinício Vieira Universidade Federal do Mato Grosso

DOI:

https://doi.org/10.36560/18420252093

Keywords:

bacteria, phosphorus, growth promotion

Abstract

The use of microorganisms to promote plant growth in agriculture is widely known. In this study, bacterial strains from swine manure-based biofertilizers, previously characterized as producers of indoleacetic acid (IAA), were evaluated for their ability to solubilize phosphate in vitro and in the growth promoting of soybean plants. The isolates were transferred to Yeast Extract (EL) medium supplemented with inorganic phosphate sources and incubated at 28ºC for 15 days. Afterwards, the presence of a transparent halo in the culture medium, indicative of phosphate solubilization, was evaluated by visual analysis. The two strains that presented the largest transparency halo around the colony were selected for the quantification test. With the use of a caliper, the transparency halo was quantified and the Average Solubilization Index (ISM) determined. With the strain with the greatest phosphate solubilization potential in vitro, a bacterial suspension was prepared in which the soybean seeds were immersed and subsequently sown in gerboxes for a period of 10 days. Four treatments were used: T1 - distilled water; T2 - saline solution (0.85% NaCl); T3 - with bacteria (1.5.108 cfu.mL-1 in saline solution); and T4 - with bacteria (9.108 cfu.mL-1 in saline solution) in four replicates (each gerbox representing one replicate, containing 25 seeds). Soybean seeds were immersed in a bacterial suspension (1.5.108 cfu.mL-1) for a period of 15 minutes and sown in pots containing non-sterilized soil. Supersimple fertilizer was used at half or full recommended dose. The experiment, carried out under greenhouse conditions, consisted of 6 treatments: T1 (no bacteria + 0% phosphate fertilizer); T2 (no bacteria + 50% phosphate fertilizer); T3 (no bacteria + 100% phosphate fertilizer); T4 (with bacteria + 0% phosphate fertilizer); T5 (with bacteria + 50% phosphate fertilizer) and T6 (with bacteria + 100% phosphate fertilizer). The collection was carried out 30 days after sowing, and the following were evaluated: plant height (AP), root volume (VR), aerial part dry mass (MSA) and root dry mass (MSR), after drying in a forced air circulation oven at 65°C for 48 hours until constant weight was obtained. Of the 13 strains tested, BS-16 stood out, presenting the highest solubilization index (0.396) in the “in vitro” tests. Although no higher germination rate was observed in soybean seeds, it was found that BS-16, at both concentrations tested (1.5,108 cfu.mL-1 and 9,108 cfu.mL-1) inhibited the reducing effect of seed germination resulting from the action of the saline solution. No growth-promoting action was observed in soybean plants (plant height, root volume and shoot biomass) in the presence of BS-16. However, in treatments involving the application of phosphate fertilizer (half and full dose) without the presence of the bacterial strain, an inhibition in root biomass production was observed. Thus, it was found that the BS-16 promotes a reduction in the inhibitory effects resulting from the use of the saline solution and in the application of a half dose of phosphate fertilizer, indicating potential use in the remediation of cultivable saline soils.

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Published

2025-06-25

How to Cite

Shiomi, H. F., Sabino, D. C. C., Serbai, D. R., Busanelo, J. D. P., & Vieira, C. V. (2025). Selecting phosphate solubilizing bacteria from suine biofertilizer. Scientific Electronic Archives, 18(4). https://doi.org/10.36560/18420252093