Avaliação do efeito biológico de Moringa oleifera Lam. in vivo

Wemerson Aparecido da Silva  Ferreira; Erica Gabriele da Silva  Pereira; Danilo Henrique  Aguiar; Angellica Fernandes de  Oliveira; Marina Mariko  Sugui

doi:10.36560/16120231731

Authors

Wemerson Aparecido da Silva Ferreira Universidade Federal de Mato Grosso – Campus Universitário de Sinop
Erica Gabriele da Silva Pereira Universidade Federal de Mato Grosso – Campus Universitário de Sinop
Danilo Henrique Aguiar Universidade Federal de Mato Grosso – Campus Universitário de Sinop
Angellica Fernandes de Oliveira Universidade Federal de Mato Grosso – Campus Universitário de Sinop
Marina Mariko Sugui Universidade Federal de Mato Grosso – Campus Universitário de Sinop

DOI:

https://doi.org/10.36560/16120231731

Keywords:

Moringa oleifera, chemoprevention, cancer

Abstract

Moringa oleifera Lam. belongs to the Moringaceae family, popularly known in Brazil as "moringa", "lírio branco" or "quiabo-de-quina", has numerous health benefits, including nutritional and medicinal properties. The study aimed to evaluate the mutagenic/antimutagenic and cytotoxic activities of the aqueous extract of M. oleifera against damage induced to DNA by N-ethyl-N-nitrosurea (ENU) in bone marrow cells of male Swiss mice, through of the micronucleus test and cell viability in Chinese Hamster Ovary (CHO) cell cultures, respectively. Eight animals/group were used, which received for 15 consecutive days the treatment, via gavage, with aqueous extract of M. oleifera. On the 15^th day, the animals received intraperitoneal treatment with 0.9% NaCl or ENU (50 mg/Kg), being sacrificed 24 hours after treatment to evaluate the frequency of micronucleated polychromatic erythrocytes (MNPCEs). For the evaluation of cytotoxic activity in vitro of CHO cells, the trypan blue exclusion assay was used for the concentrations of 5000, 2500, 1000, 200, 100 and 50 µg/mL of the extract of M. oleifera. The results obtained show that the pretreatment with the aqueous extract of the M. oleifera, under the conditions tested, significantly reduced (p ≤ 0.01) the frequency of MNPCEs induced by ENU when compared with the positive control group, presenting antimutagenic effect. There was no mutagenic effect in the group treated only with the aqueous extract of M. oleifera. The cell viability test by trypan blue showed viable cells only at the tested concentrations of 100 µg mL (85.6%) and 50 µg mL (95.5%). In this context, the antimutagenic effect presented by the aqueous extract of M. oleifera may be the result of a synergistic action between the various constituents of the plant and its cytotoxic action depends on the concentration used. Under the conditions carried out, the study suggests that the aqueous extract of M. oleifera has no cytotoxic action at low concentrations and has a chemoprotective effect for cancer.

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